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KMID : 0380620110430020224
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Korean Journal of Food Science and Technology
2011 Volume.43 No. 2 p.224 ~ p.229
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Analysis of Zearalenone Contamination in Cereal-Based Products Using High Performance Liquid Chromatography-Fluorescence Detector and Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
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Jang Mi-Ran
Choi In-Sun
Shin Choon-Shik
Kim Jin-Hee
Ahn Dong-Hyun
Lee Chang-Hee
Jang Young-Mi
KIm Dong-Sul
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Abstract
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Zearalenone (ZEA) is an estrogenic mycotoxin mainly produced by Fusarium graminerum, a species which colonizes a wide variety of cereals, including wheat, barley and processed products. A survey of ZEA contamination was conducted on 141 dried confectioneries, 59 breads and rice cakes, 135 noodles and 101 other products, for a total of 432 commercial samples. Samples were analyzed by high performance liquid chromatography with fluorescence detection (HPLC-FLD) after immunoaffinity clean-up and was confirmed by liquid chromatography tandem mass spectrometry (LCMS/ MS). The limits of detection and quantification were 2.0 and 6.0 μg/kg, respectively. The recovery ranged from 80.2% to 98.4% in the cereal based product. ZEA was detected in 38 samples (8.8% incidence), including 3 snack, 2 biscuit and 33 other cereal products. The ZEA contamination levels were in the range of 5.38-53.76 μg/kg. Finally, LC-MS/MS analysis of the contaminated samples was conducted to confirm the detected ZEA, and all 38 samples showing ZEA by HPLC-FLD were confirmed by LC-MS/MS.
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KEYWORD
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mycotoxin, zearalenone, immunoaffinity column, cereal
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